Regulatory

Part:BBa_K2057000:Design

Designed by: Uriel Eleazar Barboza Pérez   Group: iGEM16_Guanajuato_Mx   (2016-10-14)

(I) Additional information about the utility of the promoter of chiA74 to drive the expression of the endochitinase in E. coli is found in the following manuscripts:

(1) BARBOZA-CORONA JE, Delgadillo-Ángeles JL, Castañeda-Ramíırez JC, Barboza-PĂ©rez UE, Casados-VĂĄzquez LE, Bideshi DK and del RincĂłn-Castro MC (2014) Bacillus thuringiensis subsp. kurstaki HD1 as a factory to synthesize alkali-labile ChiA74∆sp chitinase inclusions, Cry crystalsand spores for applied use. Microb Cell Fact 13, 15. doi: 10.1186/1475-2859-13-15 (2) BARBOZA-CORONA JE, Nieto-Mazzocco E, VelĂĄzquez-Robledo R, Salcedo-Hernandez R, Bautista M, JimĂ©nez B, Ibarra JE (2003) Cloning, sequencing and expression of the chitinase chiA74 from Bacillus thuringiensis.Appl Environ Microbiol. 2003 Feb;69(2):1023-9. (II) THE WHOLE SEQUENCE of the chiA74 gene including its regulatory elements (promoter, RBS, transcriptional terminator) is found under accession number AF424979 (GenBank nucleotide sequence database).